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. 2021 Feb 5;22(4):1619. doi: 10.3390/ijms22041619

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of Kaempferol, present in two different Cu–Kaempferol molar ratios (5:1 and 1:1), on the formation of free radicals by means of a copper-catalyzed Fenton reaction. (a) Experimental EPR spectra obtained in the system CuCl₂/H₂O₂/DMPO/DMSO–H₂O (1:4; v:v) under an air atmosphere, without (Ref) or with Kaempferol. (b) The total concentration of DMPO spin adduct concentration monitored in DMSO/water (1:4; v:v) solution of CuCl₂ alone (Ref) or in the presence of Kaempferol containing DMPO spin trapping agent after the addition of hydrogen peroxide. Initial concentrations: c(CuCl₂) = 0.2 mM; c(Kaempferol) = 0/0.04/0.2 mM; c(DMPO) = 0.02 M; c(H₂O₂) = 0.01 M. EPR parameters: ^•DMPO-OH (a_N = 1.486 mT, a_H^β = 1.438 mT; g = 2.0057); ^•DMPO-O₂^– (a_N = 1.464 mT, a_H^β = 1.138 mT, a_H^γ = 0.193 mT; g = 2.0057); ^•DMPO-CH₃ (a_N = 1.558 mT, a_H^β = 2.232 mT; g = 2.0056).