Figure 9.

Electrophoretic profiles of agarose gel (0.8%) containing 15 µmol plasmid DNA pBSK+ with Cu(II) ions and hydrogen peroxide, to which 10 µmol and 200 µmol of kaempferol had been added, respectively. Samples were loaded in the gel lanes in the following order: (p) pDNA-control; Left panel: (1) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 10 µM kaempferol, (2) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 10 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 10 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 10 µM kaempferol + SOD; Right panel: (1) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 200 µM kaempferol; (2) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 200 µM kaempferol + L-histidine; (3) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 200 µM kaempferol + DMSO; (4) pDNA + 5 µM CuCl₂ + 50 µM H₂O₂ + 200 µM kaempferol + SOD. (L) 1 kb DNA standard, (lin) pDNA linearized with EcoR1 endonuclease, (µM = µmol.dm⁻³).