Figure 5.

Concentration of 8-iso-Prostaglandin F2α in peripheral mononuclear blood cells (PBMCs) culture supernatant. Results are expressed as geometric estimated means ± SEM. CON = Untreated PBMCs; PMA = PBMCs treated with 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 $\mu$g/mL ionomycin; PMA + AAPH = PBMCs treated with 10 ng/mL PMA, 1 $\mu$g/mL ionomycin, and 3 mM of 2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH); AAPH = PBMCs treated with 3 mM AAPH; BHV = PBMCs treated 8 × 10⁴ TCID₅₀ Bovine Herpesvirus 1 per 1 × 10⁶ PBMCs; BHV + AAPH = PBMCs treated 8 × 10⁴ TCID₅₀ Bovine Herpesvirus 1 per 1 × 10⁶ PBMCs and 3 mM AAPH. Differences between treatments and the control were assessed with Dunnett’s pairwise post hoc test * p < 0.05, ** p < 0.01, *** p < 0.001.