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. 2021 Feb 7;10(2):255. doi: 10.3390/antiox10020255

Figure 9.

Figure 9

Relative expression of the genes (a) Interferon Gamma (IFNG), (b) Interleukin 2 (IL2), (c) Interleukin 4 (IL4), and (d) Interleukin 10 (IL10) in peripheral mononuclear blood cells (PBMCs) of newborn calves. Results are normalized to the gene expression of the negative control (CON). PMA = PBMCs treated with 10 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin; PMA + AAPH = PBMCs treated with 10 ng/mL PMA, 1 µg/mL ionomycin, and 3 mM of 2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH); AAPH = PBMCs treated with 3 mM AAPH; BHV = PBMCs treated 8 × 104 TCID50 Bovine Herpesvirus 1 per 1 × 106 PBMCs; BHV + AAPH = PBMCs treated 8 × 104 TCID50 Bovine Herpesvirus 1 per 1 × 106 PBMCs and 3 mM AAPH. Results are expressed as estimated means ± SEM of relative quantity (RQ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns: p > 0.05.