Figure 5.

Morphometric analysis of the signs of microglial activation in the wild type group and the SOD1G93A group. (A–C) The number of Iba-1+ cells per area of 0.1502 mm² in the OS (A), OPL (B), and ILC (C); (D,E) Quantitative analysis of the area of the microglial cells (µm²) in OPL (D) and ILC (E). (F–G) Quantitative analysis of the arbor area of the microglial cells (µm²) in OPL (F) and ILC (G). The histograms show the mean number (± standard deviation, SD). * p < 0.05, ** p < 0.01. Photoreceptor outer segment layer (OS), outer plexiform layer (OPL), and inner layer complex (ILC) (constituted by an inner plexiform layer and a nerve fiber–ganglion cell layer). Wild type (WT). Superior (SUP), inferior (INF), nasal (NAS), and temporal (TEM). Number of retinas used in the experiment, WT: n = 6; and SOD1G93A: n = 6. Statistical test: Mann–Whitney U test.