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. 2021 Feb 4;10(2):324. doi: 10.3390/cells10020324

Figure 6.

Figure 6

Knock-down of HCN-channel subunit 4 speeds up the inward rectification (sag) and Ih. (Aa) Representative current traces of whole-cell patch-clamp recordings from −130 mV current pulses of HEK293 cell lines (left) and wildtype (wt) or sh4-/shScr-transduced primary hippocampal neurons (PHNs; right). (Ab) Time constants of monoexponential fits from the current responses of the HEK293 cell-lines (left) and wt or sh4-/shScr-transduced PHNs (right). (Ba) Representative voltage traces of whole-cell patch-clamp recordings from current pulses which hyperpolarize the membrane potential to −130 mV of HEK293 cells stably expressing HCN-channel subunit 1, 2, or 4 (left) and wt or rAAV9-transduced eGFP-positive PHNs (right). (Bb) Sag half-width of HEK293 cell-lines (left) and wildtype or transduced PHNs (right). Results are depicted as boxplots. Statistical significance was assessed using the unpaired two-tailed Student’s t test, * p < 0.05, ** p < 0.01.