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. 2021 Feb 9;9(2):170. doi: 10.3390/biomedicines9020170

Figure 6.

Figure 6

Concentration of selected cytokines in supernatants of regulatory T cells (Treg)/conventional T cells (Tconv) cocultures. Non-Treg CD4+ T cells (Tconv) magnetically isolated from children of healthy (n = 14) and allergic (n = 15) mothers were cultured with IL-2, unstimulated (negative control) or stimulated with a cocktail of functional-grade anti-CD3 + anti-CD28 antibodies (positive control). Treg magnetically isolated from the same cord blood samples were added to stimulated Tconv at 1:5 Treg/Tconv ratio. Culture supernatants were collected after 72 h of culture and concentrations of selected cytokines in the supernatant were measured with ELISA. (a) Concentrations of IL-10 in the supernatant of unstimulated Tconv, stimulated Tconv and Treg/Tconv cocultures of Tconv and Treg of children of healthy and allergic mothers. * p < 0.05, ** p < 0.01, *** p < 0.001. (b) Concentrations of TGF-β in the supernatant of unstimulated Tconv, stimulated Tconv and Treg/Tconv cocultures of Tconv and Treg of children of healthy and allergic mothers. (c) Concentrations of TNF-α in the supernatant of unstimulated Tconv, stimulated Tconv and Treg/Tconv cocultures of Tconv and Treg of children of healthy and allergic mothers. * p < 0.05.