Figure 1.

J.CaM1.7 cells are double negative for LAT and Lck expression. (A) J.CaM1.7 cells derived from J.CaM1.6 cells were analyzed for LAT, Lck, and β-actin expression by Western blot. Lysates obtained from 10⁶ Jurkat, J.CaM2 (LAT deficient), J.CaM1.6 (Lck deficient), and J.CaM1.7 cells were analyzed by Western blot with the indicated specific antibodies. (B) RT–PCR analysis of Jurkat, J.CaM2, J.CaM1.6, and J.CaM1.7 cells with specific primers for LAT and HPRT1. For each cell line, a negative control has been included in which RNA was treated without reverse transcriptase. (C) LAT relative mRNA levels determined by qPCR analysis in the indicated cell lines and normalized against HPRT1. Values are displayed as means ± s.d. of three independent biological replicates. Asterisks (*) represent statistical significance (p < 0.001).