Figure 2.

Stability and induction of LAT expression in J.CaM1.6 and J.CaM1.7 cells. (A) J.CaM1.6 cells were grown at 37 °C and 5% CO₂ continuously after thawing for the indicated weeks, and then cell lysates were obtained and analyzed for LAT, Lck, and β-actin expression by Western blot. Lysates obtained from Jurkat and J.CaM2 were incorporated as positive and negative controls. (B) J.CaM2, J.CaM1.6, and J.CaM1.7 cells were unstimulated or PMA treated (20 ng/mL) in the absence or presence of ionomycin (Iono; 3 μM). Whole-cell lysates were prepared 24 h post-stimulation and immunoblotted with antibodies specific for LAT, Lck, and Erk. (C) LAT protein relative expression determined by densitometric quantification of Western blot in subfigure (B) normalized against Erk expression. Data in subfigures (B,C) are representative of three biological replicates. NT, not treated; P, PMA; I, ionomycin; P + I, PMA + ionomycin.