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Immunofluorescence staining of the four antigens: PLA2R, THSD7A, Sema3B, NELL-1. Paraffin embedded tissue biopsies were stained after retrieval with the relevant antibodies. Fine granular staining of the immune deposits is seen on the outer aspect of the glomerular basement membrane. Note that some capillary loops are not, or weakly stained with anti-NELL-1 antibody.
