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. 2021 Feb 4;10(2):241. doi: 10.3390/antiox10020241

Figure 5.

Figure 5

Effects of NAC or Trolox on proliferation of human colon cancer cell lines. (A) Real-time analyses of proliferation of two human colon cancer cell lines, LoVo and Colo320, cultured in medium supplemented with 200 μM NAC or 20 μM Trolox compared to untreated control (NAC) or DMSO control (Trolox); data are means of six replicates per cell line. (B) Graphs showing relative intensity of DCFH-DA, DHR, and DHE probes in LoVo and Colo320 cells, cultured as described above (n = 6). DCFH-DA, dichloro-dihydro-fluorescein diacetate; DHR, dihydrorhodamine; DHE, dihydroethidium; a.u., arbitrary units; error bars indicate SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.