Table 1.
Overview of the output statistics. The sequencing and assembly quality of the venom gland transcriptome of Hydrophis curtus.
| Parameter | Output Statistics |
|---|---|
| Total raw reads | 57,606,566 |
| Total clean reads | 54,140,326 |
| Total clean nucleotides (nt) | 4,872,629,340 |
| Q20 percentage | 98.60% |
| N percentage | <0.01% |
| GC percentage | 44.63% |
| Contigs created | 126,790 |
| Total length (nt) | 51,459,117 |
| Mean length (nt) | 406 |
| N50 | 921 |
| Unigenes/transcripts assembled | 82,209 |
| Total length (nt) | 69,679,280 |
| Mean length (nt) | 848 |
| N50 | 2073 |
| Unigene/transcripts assembled (FPKM > 1) | 70,564 |
| Unidentified | 45,616 (17.97%) |
| -Redundancy (FPKM abundance/number of transcripts) | 3.8 |
| Non-toxin | 24,852 (33.86%) |
| -Redundancy (FPKM abundance/number of transcripts) | 13.16 |
| Toxin | 96 (48.18%) |
| -Redundancy (FPKM abundance/number of transcripts) | 4847.54 |
Q20 percentage is the proportion of nucleotides with a quality value larger than 20; N percentage is the proportion of unknown nucleotides in clean reads; GC percentage is the proportion of guanidine and cytosine nucleotides among total nucleotides; N50 is the shortest contig length needed to cover 50% of the transcriptome; FPKM stands for Fragments Per Kilobase of transcript per Million mapped reads; Redundancy refers to the abundance of expression per gene transcript.