. 2021 Feb 5;22(4):1624. doi: 10.3390/ijms22041624

Table 1.

Summary of microRNAs (miRNAs) regulating colorectal cancer stem cell (CSC) growth and/or stemness.

miRNA	Expression in CSCs	Relation to Stemness	Reference
let-7f-1-3p	Downregulated	Downregulated in CD44v6⁺ cells	[42]
let-7f-1-3p	Downregulated	Downregulated in 5-fluorouracil (5-FU) and oxaliplatin-resistant CRC¹	[42]
miR-10b	Downregulated	Downregulated in ALDH⁺/CD44⁺ cells	[47]
miR-15a	Downregulated	Targets BCL2, BMI1, YAP1 and DCLK1	[50]
miR-15a	Downregulated	5-FU-modified mimic promotes apoptosis, inhibits invasion and overcomes resistance in CSC ¹	[50]
miR-18a	Downregulated	Upregulated in CRC, but downregulated in CRC CSCs	[47]
miR-20b-5p	Downregulated	Inhibits proliferation of spheroid cells, invasion and tumorigenic capacity	[51]
miR-34a-3p	Downregulated	Downregulated in CD44v6⁺ cells	[42]
miR-34a-3p	Downregulated	Downregulated in 5-FU and oxaliplatin-resistant CRC	[42]
miR-101-3p	Downregulated	Downregulated in CD44v6⁺ cells	[42]
miR-101-3p	Downregulated	Downregulated in 5-FU and oxaliplatin-resistant CRC	[42]
miR-129	Downregulated	Promotes apoptosis and inhibits proliferation	[52,53]
		Targets BCL2 and E2F3
		Enhances sensitivity to 5-FU ¹
		5-FU-modified mimic promotes apoptosis, inhibits invasion and overcomes resistance in CSC ¹
miR-133b	Downregulated	Downregulated in CD44⁺/CD133⁺/CD24⁺/CD166⁺ cells	[54]
		Targets DOT1L which promotes expression of OCT-3/4, NANOG, and SOX2
		Enhances sensitivity to 5-FU and oxaliplatin ¹
miR-139-5p	N/A	Overexpression leads to loss of stemness in CD133⁺/CD44⁺ CSCs	[55]
miR-139-5p	N/A	Targets E2-2 which promotes Wnt signaling pathway	[55]
miR-140-5p	Downregulated	Inhibits proliferation of spheroid cells and invasion	[56]
		Targets Smad2 which promotes TGF-β pathway
		Targets ATG12 which promotes autophagy
miR-148a	Downregulated	Promotes apoptosis	[57]
miR-148a	Downregulated	Enhances sensitivity to cisplatin ¹	[57]
miR-200c-3p	Downregulated	Downregulated in CD44v6⁺ cells	[42]
miR-200c-3p	Downregulated	Downregulated in 5-FU and oxaliplatin-resistant CRC	[42]
miR-210	Upregulated	Upregulated in ALDH⁺ and CD44⁺ cells	[47,48]
miR-221	Upregulated	Upregulated in ALDH⁺/CD44⁺ and EpCAM⁺/CD44⁺ cells	[47,49]
		Overexpression leads to enhanced formation of 3D organoids
		Targets QKI which regulates LGR5, SOX2 and OCT-4
		Anti-miR-221 inhibits CRC proliferation and promotes apoptosis
miR-302a	Downregulated	Downregulated in cetuximab-resistant CRC ¹	[58]
miR-302a	Downregulated	Targets CD44	[58]
miR-4711-5p	N/A	Inhibits proliferation of spheroid cells	[59]
miR-4711-5p	N/A	Targets CD44v9, LGR5, BMI1 and KLF5	[59]

¹ These miRNAs have demonstrated therapeutic potential.