Figure 6.

Mechanism of cell death by the ethanolic extract of Euphorbia lathyris. (A) Western blot analysis of expression of procaspase and cleaved caspase 3, 8, and 9 in T84 cells before treatment (a) and after exposure to the ethanolic extract during 12 h with IC50 dose (b), 12 h with twice IC50 dose (c), 24 h of treatment with IC50 dose (d), and 24 h with twice IC50 dose (e). (B) Graphic representation of Western blot densitometry analysis of bands corresponding to coefficient between cleaved caspase and procaspase 3, 8, and 9 expression after treatment with the ethanolic extract (IC50 dose and twice IC50 dose) at different time (12 h and 24 h) (b–e) compared to the untreated control (a). Data are presented as mean ± standard deviation of three independent experiments; * p < 0.05 vs. control group; ** p < 0.01 vs. control group. (C) Representative microscopy images of fluorescence emission from lysosomal and α-tubulin probing of T84 cell line exposed to the ethanolic extract. Formation of autophagic vesicles was evident in the treated cells (arrows).