Skip to main content
. 2021 Feb 8;22(4):1711. doi: 10.3390/ijms22041711

Figure 5.

Figure 5

(A) Schematic diagram of dual-luciferase plasmids constructed with Wt-VEGF, Del-VEGF and Mut-VEGF i-motif-forming sequences of the VEGF promoter. (B) Effect of 3c on Wt-VEGF, Del-VEGF, and Mut-VEGF promoter determined by using dual luciferase reporter assays. (C) The mRNA levels of VEGF, BCL2, KRAS, c-kit, PDGFR-β in MCF-7 cells were analyzed by using qRT-PCR after incubation with increasing concentration of 3c. (D) Western blot results of VEGF protein in MCF-7 cells in the presence of increasing concentrations of 3c from 0 to 6 μM. The GAPDH protein was used as a control. The experiments were repeated for three times. The data are expressed as the mean ± SEM: (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, (****) p < 0.0001.