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. 2021 Feb 4;13(4):621. doi: 10.3390/cancers13040621

Figure 2.

Figure 2

IGF-1 induces the expression of S100A7. (A) mRNA expression of S100A7 in MCF-7 and T47D cells treated vehicle or IGF-1 (10 nM, 48 h), as evaluated by qRT-PCR. Values were normalized to the 36B4 gene expression and shown as fold changes of mRNA expression induced by IGF-1 compared to cells treated with vehicle. Data are mean ± SEM of at least three independent experiments performed in duplicate. (B) Transactivation of a S100A7 (pS100A7) promoter construct observed in MCF-7 and T47D cells treated with IGF-1 (10 nM, 18 h). Luciferase activity was normalized to the internal transfection control. Results are expressed as the % change of normalized RLU values relative to vehicle-treated cells. Data shown are the mean ± SEM of at least two independent experiments performed in triplicate. (C,D) Representative immunoblots showing the increase of S100A7 protein expression in MCF-7 and T47D cells treated with IGF-1 (10 nM, 48 h). β-actin serves as loading control. Lower panels show densitometric analysis of the blots normalized to β-actin. Data shown are the mean ± SEM of at least two independent experiments. (*) p < 0.05; (**) p < 0.01.