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. 2021 Feb 4;13(4):621. doi: 10.3390/cancers13040621

Figure 6.

Figure 6

Paracrine release of S100A7 by BC cells induces proliferative effects in HUVECs. (A) Evaluation of S100A7 concentration in conditioned medium from MCF-7 cells treated with vehicle or IGF-1 (10 nM, 48 h), as evaluated by ELISA. Data shown are the mean ± SEM of at least three independent experiments performed in duplicate. (B) HUVECs were cultured in medium collected from MCF-7 cells treated with either vehicle or IGF-1 (10 nM, 48 h), in the presence of the RAGE inhibitor FPS-ZM1 (2 μM). Thereafter, HUVECs were subjected to SRB assay for the evaluation of cell growth. (C) HUVECs were cultured in 0.1% FBS in the presence of vehicle and human recombinant S100A7 (0.15μg/mL), alone and in combination with the RAGE inhibitor FPS-ZM1 (2 μM), as indicated. After 48 h, HUVECs were subjected to SRB assay for the evaluation of cell growth. Data shown are the mean ± SEM of at least 2 independent experiments performed in sextuplicate. (*) p < 0.05; (***) p < 0.001.