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. 2021 Feb 7;10(2):254. doi: 10.3390/antiox10020254

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Exogenous NAD⁺ restores NAD⁺ levels in senescent BMSCs. BMSCs were induced by 10 g/L of D-gal for 48 h and then were treated by different concentrations of exogenous NAD⁺ for another 48 h. (a) A NAD⁺/NADH content colorimetric kit was used to detect the changes of NAD⁺ levels in senescent BMSCs. (b) Changes of NAD⁺/NADH in senescent BMSCs determined by the NAD⁺/NADH content colorimetric kit. The data are expressed as means ± standard deviation. n = 3; * p < 0.05; ** p < 0.01. BMSCs: Bone marrow-derived mesenchymal stem cells; D-gal: D-galactose; NAD⁺: Nicotinamide adenine dinucleotide.