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. 2021 Feb 7;10(2):254. doi: 10.3390/antiox10020254

Figure 5.

Figure 5

Exogenous NAD+ regulates the expression of Sirt1 and PARP1 in senescent BMSCs. BMSCs were induced by 10 g/L of D-gal for 48 h and were treated by different concentration of exogenous NAD+ for another 48 h. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of sirt1 (a) and parp1 (b) in senescent BMSCs. Western blotting (WB) was used to detect the expression of Sirt1 (c) and PARP1 (d) in senescent BMSCs, and the values below the bands are their quantitative results. The data are expressed as means ± standard deviation. n = 3; * p < 0.05; ** p < 0.01; *** p < 0.001. BMSCs: Bone marrow-derived mesenchymal stem cells; D-gal: D-galactose; NAD+: Nicotinamide adenine dinucleotide; Sirt1: Sirtuin 1; PARP1: Poly (ADP-ribose) polymerase 1.