Figure 1.

ALCL plasmatic small extracellular vesicle (S-EV) characterization. (a) Transmission electron microscopy analysis of plasmatic S-EVs from a representative ALCL sample. (b) Western blotting analysis of peripheral blood mononuclear cells (PB-MNCs) and representative ALCL plasmatic S-EVs, showing the presence of heat shock protein 90-kDa (HSP90) protein in both whole cells and S-EV lysates, exosome-specific tetraspanin CD9 in S-EV lysates only and the endoplasmic reticulum marker Calnexin (CANX) in total lysates, but not in S-EVs. (c) Size distribution of particle diameters (nm) of vesicles isolated from a representative ALCL plasma sample, as measured by nanoparticle tracking analysis (Nanosight, Malvern Panalytical).