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. 2021 Feb 9;22(4):1715. doi: 10.3390/ijms22041715

Figure 3.

Figure 3

Stress treatment enhanced the signal of LkDHNs in nucleus. (A) The effect of salt stress on the nuclear localization of LkDHNs was observed by a Leica SP8 laser confocal microscope. a and b are GFP with 0 and 200 mM NaCl, respectively; c and d are LkDHN1 with 0 and 200 mM NaCl, respectively; e and f are LkDHN2 with 0 and 200 mM NaCl, respectively; g and h are LkDHN3 with 0 and 200 mM NaCl, respectively; i and j are LkDHN4 with 0 and 200 mM NaCl, respectively. (B) Analysis of GFP fluorescence signal in nucleus under salt stress. a: GFP; b: LkDHN1; c: LkDHN2; d: LkDHN3; e: LkDHN4. The purple line represents the fluorescence after 30 min treatment with 200 mM NaCl, and the blue line represents the fluorescence under 0 mM NaCl. The nucleuses were indicated by red arrow.