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. 2021 Feb 9;22(4):1715. doi: 10.3390/ijms22041715

Figure 5.

Figure 5

LkDHNs increased the tolerance of tobacco protoplasts and yeasts to osmotic stress. (A) The tolerance of tobacco protoplasts with LkDHNs to osmotic stress was enhanced. a and b are the enzymatic hydrolysis of GFP at 0.4 and 0.6 M mannitol, respectively; c and d are the enzymatic hydrolysis of LkDHN1 at 0.4 and 0.6 M mannitol, respectively; e and f are the enzymatic hydrolysis of LkDHN2 at 0.4 and 0.6 M mannitol, respectively; g and h are the enzymatic hydrolysis of LkDHN3 at 0.4 and 0.6 M mannitol, respectively; i and j are the enzymatic hydrolysis of LkDHN4 at 0.4 and 0.6 M mannitol, respectively. The intact protoplasts were indicated by red arrow; the broken protoplasts were indicated by blue arrow. The scale bar is 500 μm. (B) Enhanced tolerance of yeast overexpressing LkDHNs to osmotic stress. a: spot assay of pPIC9 and LkDHNs on YPD medium; b: spot assay of pPIC9 and LkDHNs on YPD medium with 2 M sorbitol; c: spot assay of pPIC9 and LkDHNs on YPD medium with 1.5 M NaCl.