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. 2021 Feb 7;22(4):1661. doi: 10.3390/ijms22041661

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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OsbZIP09 controls seed germination by suppressing the expression of Late Embryogenesis Abundant (LEA) family genes and enhancing the expression of OsLOX2. (A) OsbZIP09-binding motif analysis in the promoter of LEA25 and LOX2, respectively. (B) Schematic depiction of the reporter and effector constructs used in the dual-luciferase reporter assay. (C,D) OsbZIP09 suppresses the promoter activity of LEA25 but activates the promoter activity of LOX2. Data are shown as means ± SD (n = 5). Statistically significant differences at p < 0.05 are indicated by different letters.