Figure 5.
OsCRP1 overexpression in rice confers drought tolerance. (a) Phenotypes of OsCc1::OsCRP1OX and OsCc1::OsCRP1RNAi transgenic rice plants under drought stress at the vegetative stage. Three independent homozygous OsCc1::OsCRP1OX and OsCc1::OsCRP1RNAi lines and NT control plants were grown in soil for 5 weeks and exposed to drought for 3 days, followed by re-watering. (b) Soil moisture in the pots exposed to drought treatment at the indicated time points. Values are the means ± SD (n = 10). (c) Chlorophyll fluorescence (Fv/Fm) of OsCc1::OsCRP1OX and OsCc1::OsCRP1RNAi transgenic rice plants and NT plants during a 3-day drought treatment. Fv/Fm values were measured in the dark to ensure sufficient dark adaptation. Data are shown as the mean ± SD (n = 30). (d) ATP contents in leaves of the OsCc1::OsCRP1OX, NT and OsCc1::OsCRP1RNAi plants under drought stress conditions. Five-week-old plants were transferred to water for 2 days prior to drought treatments. Air-dried plants were taken at 1 and 3 h of treatment to compare ATP contents. Ten plants were used for each line. Data bars represent the mean ± SD of three biological replicates, each of which had two technical replicates. Asterisks indicate significant differences compared with NT (* p < 0.05, One-way ANOVA).