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. 2021 Feb 8;13(4):687. doi: 10.3390/cancers13040687

Figure 1.

Figure 1

NOX4 mediates HKMT (heat-killed Mycobacterium tuberculosis) -induced collagen synthesis via POLDIP2 and autophagy signaling: Human Met5A cells were transfected with siRNA targeting NOX4 (SiNOX4). After 1 day, Met5A cells were treated with HKMT (10 ng/mL) for 1 h or untreated (A–D). NOX4 mediates autophagy signaling in BCG (bacillus Calmette-Guérin)-induced lung fibrosis (E,F). (A) RT-PCR analysis ofNOX4, POLDIP2, and COL1A mRNA levels. (B) Cytokine levels (TGF-β, IL-6, and TNF-a) were assessed by ELISA in conditioned media of Met5A cells following transfection with siCon or siRNA targeting NOX4 (SiNOX4). (C) Western blotting of p62, LC3II/LC3I, collagen, POLDIP2, and NOX4. (D) LC3-puncta formation with quantification in Met5A cells with different treatments. Images were obtained by confocal microscopy (80×). Scale bar: 50 μm. (E) Western blotting of ATG12-ATG5 and LC3B. (F) Immunostaining of p62 in mouse lungs (200×). Scale bar: 50 μm. Results are presented as the mean ± SEM of at least three independent experiments. *** p < 0.001, ** p < 0.01, * p < 0.05.