Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 5;22(4):1613. doi: 10.3390/ijms22041613

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the author.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Subunit composition of purified SM deacylase recombinant human aCDase and separated recombinant β-subunit of human aCDase. The samples were separated by SDS-PAGE followed by immunoblot analysis using antibodies to the β-subunit (human) of aCDase. Before electrophoresis, samples were reduced with 5% 2-mercaptoethanol (ME) indicated by ME+ but were not treated with ME indicated by ME-. Lane 1, purified rat SM deacylase (ME+); Lane 2, recombinant human aCDase (ME+); Lane 3, recombinant human aCDase (ME-). Lane 4, mock transfected (ME-); Lane 5, separated recombinant β-subunit of human aCDase (ME-) [71].