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. 2021 Feb 9;10(2):268. doi: 10.3390/antiox10020268

Table 2.

Effect of human amniotic membrane-derived mesenchymal stem cell conditioned medium (AMSC-CM) on in vitro fertilized mouse embryos development.

Group No. of Cultured Embryos No. of Embryos Developed to (%)
4-Cell 16-Cell Blastocyst Hatched Blastocyst
Control 72 66 (91.8 ± 1.8) b 51 (71.1 ± 2.6) ab 28 (38.6 ± 4.5) a 14 (19.4 ± 4.6) a
10% AMSC-CM 74 69 (92.5 ± 4.8) b 55 (74.3 ± 4.8) b 39 (51.7 ± 4.1) b 25 (32.6 ± 5.9) b
20% AMSC-CM 74 69 (93.4 ± 3.0) b 45 (61.2 ± 3.5) a 23 (30.7 ± 5.5) a 15 (19.1 ± 3.5) a
50% AMSC-CM 76 61 (79.6 ± 4.0) a 46 (59.5 ± 3.8) a 24 (28.3 ± 6.8) a 16 (18.8 ± 3.4) a

Experiments were repeated at least 3 times. a,b Mean ± SEM with different superscript letters indicate significant differences (at least p < 0.05).