Table 4.
Putative genes of Diabrotica undecimpunctata howardi nudivirus (DuhNV).
| ORF | Gene/Domain | Length (aa) | OrNV Homologs |
|---|---|---|---|
| Replication, Repair and Recombination of DNA | |||
| DuhNV_F1_ORF5 * | Dnapol | 695 | OrNV_gp001 |
| DuhNV_F2_ORF1 * | Dnapol | 633 | OrNV_gp001 |
| DuhNV_F3_ORF23 | Dnahel | 1390 | OrNV_gp034 |
| DuhNV_F2_ORF23 | dnaint/rec | 389 | OrNV_gp075 |
| DuhNV_F2_ORF33 | lef-3 | 177 | OrNV_gp059 |
| DuhNV_F4_ORF10 | dnahel2 | 839 | OrNV_gp108 |
| DuhNV_F4_ORF20 | Dnalig | 423 | OrNV_gp121 |
| Nucleotide Metabolism | |||
| DuhNV_F2_ORF40 | rr1 | 1045 | OrNV_gp051 |
| DuhNV_F4_ORF2 | rr2 | 358 | OrNV_gp102 |
| DuhNV_F1_ORF2 | tk/GrBNV_gp17-like | 648 | OrNV_gp137 |
| DuhNV_F3_ORF31 | tk/GrBNV-gp74_like | 197 | OrNV_gp044 |
| DuhNV_F4_ORF16 | tk(guanosine monophosphate kinase) | 321 | OrNV_gp117 |
| DuhNV_F5_ORF1 | tk/GrBNV_gp044_like | 352 | OrNV_gp125 |
| Transcription | |||
| DuhNV_F3_ORF11 | p47 | 324 | OrNV_gp020 |
| DuhNV_F3_ORF30 | lef-4 | 417 | OrNV_gp042 |
| DuhNV_F2_ORF39 | lef-5 | 82 | OrNV_gp052 |
| DuhNV_F2_ORF28 | lef-8 | 988 | OrNV_gp064 |
| DuhNV_F2_ORF9 | lef-9 | 505 | OrNV_gp096 |
| DuhNV_F3_ORF21 | vlf-1 | 504 | OrNV_gp030 |
| Oral Infectivity | |||
| DuhNV_F5_ORF2 | p74 | 712 | OrNV_gp126 |
| DuhNV_F2_ORF32 | pif-1 | 489 | OrNV_gp060 |
| DuhNV_F3_ORF6 | pif2 | 376 | OrNV_gp017 |
| DuhNV_F4_ORF9 | pif-3 | 202 | OrNV_gp107 |
| Packaging, Assembly, and Morphogenesis | |||
| DuhNV_F3_ORF5 | polh/gran | 404 | OrNV_gp016 |
| DuhNV_F3_ORF22 | 19k/pif-5 | 281 | OrNV_gp033 |
| DuhNV_F2_ORF26 | Ac68 | 136 | OrNV_gp072 |
| DuhNV_F2_ORF18 | 38k | 276 | OrNV_gp087 |
| Inhibition of Apoptosis | |||
| DuhNV_F4_ORF7 | iap-3 | 202 | OrNV_gp134 |
| Unknown Function | |||
| DuhNV_F2_ORF5 | Ac81 | 173 | OrNV_gp004 |
| DuhNV_F3_ORF4 | vp39 | 250 | OrNV_gp015 |
| DuhNV_F4_ORF6 | vp91 | 695 | OrNV_gp106 |
| DuhNV_F4_ORF12 | Ac92 | 410 | OrNV_gp113 |
| DuhNV_F4_ORF14 | odv-e56 | 420 | OrNV_gp115 |
| Other | |||
| DuhNV_F1_ORF1 | ND | 240 | OrNV_gp129/gp136 |
| DuhNV_F1_ORF3 | ND | 149 | OrNV_orfC20 |
| DuhNV_F1_ORF4 | ND | 594 | OrNV_gp138 |
| DuhNV_F2_ORF2 | trypsin-like serine protease-like protein | 339 | OrNV_gp002 |
| DuhNV_F2_ORF3 | trypsin-like serine protease-like protein | 427 | OrNV_gp002 |
| DuhNV_F2_ORF4 | ND | 395 | OrNV_gp003 |
| DuhNV_F2_ORF6 | thymidylate synthase/pyrimidine hydroxymethylase-like | 296 | OrNV_gp006 |
| DuhNV_F2_ORF7 | ND | 143 | OrNV_gp098 |
| DuhNV_F2_ORF8 | mRNA decapping enzyme 2-like | 245 | OrNV_gp097 |
| DuhNV_F2_ORF10 | ND | 314 | OrNV_gp095 |
| DuhNV_F2_ORF11 | ND | 162 | OrNV_gp094 |
| DuhNV_F2_ORF12 | cl17238, RING-Ubox | 329 | no hit |
| DuhNV_F2_ORF13 | None | 62 | no hit |
| DuhNV_F2_ORF14 | None | 63 | no hit |
| DuhNV_F2_ORF15 1 | cl25745, pfam05764 | 1412 | OrNV_gp090 |
| DuhNV_F2_ORF16 | None | 148 | no hit |
| DuhNV_F2_ORF17 | None | 78 | no hit |
| DuhNV_F2_ORF19 | ND | 189 | OrNV_gp086 |
| DuhNV_F2_ORF20 | ND | 227 | OrNV_gp080 |
| DuhNV_F2_ORF21 | ND | 89 | OrNV_gp079 |
| DuhNV_F2_ORF22 | ND | 52 | OrNV_gp076 |
| DuhNV_F2_ORF24 | None | 61 | no hit |
| DuhNV_F2_ORF25 | ND | 593 | OrNV_gp073 |
| DuhNV_F2_ORF27 | None | 56 | no hit |
| DuhNV_F2_ORF29 | ND | 74 | OrNV_gp063 |
| DuhNV_F2_ORF30 | ND | 88 | OrNV_gp062 |
| DuhNV_F2_ORF31 | ND | 140 | OrNV_gp061 |
| DuhNV_F2_ORF34 | HZV_115-like protein | 425 | OrNV_gp058 |
| DuhNV_F2_ORF35 | patatin-like phospholipase-like protein | 397 | OrNV_gp057 |
| DuhNV_F2_ORF36 | ND | 576 | OrNV_gp056 |
| DuhNV_F2_ORF37 | ND | 458 | OrNV_gp054 |
| DuhNV_F2_ORF38 | ND | 234 | OrNV_gp053 |
| DuhNV_F3_ORF1 | mitochondrial carrier protein-like protein | 293 | OrNV_gp011 |
| DuhNV_F3_ORF2 | ND | 231 | OrNV_gp013 |
| DuhNV_F3_ORF3 | None | 76 | no hit |
| DuhNV_F3_ORF7 | None | 388 | no hit |
| DuhNV_F3_ORF8 | ND | 131 | OrNV_orfC1 |
| DuhNV_F3_ORF9 | ND | 509 | OrNV_gp018 |
| DuhNV_F3_ORF10 | Ac46 | 89 | OrNV_gp019 |
| DuhNV_F3_ORF12 2 | EXL15_gp12_like/DUF4679 (pfam15728) | 432 | no hit |
| DuhNV_F3_ORF13 | ND | 241 | OrNV_gp022 |
| DuhNV_F3_ORF14 | guanylate kinase-like protein | 245 | OrNV_gp023 |
| DuhNV_F3_ORF15 | ND | 196 | OrNV_gp024 |
| DuhNV_F3_ORF16 | ND | 550 | OrNV_gp025 |
| DuhNV_F3_ORF17 3 | B2K52_gp057/Smc cl341474 | 858 | no hit |
| DuhNV_F3_ORF18 | ND | 422 | OrNV_gp027 |
| DuhNV_F3_ORF19 4 | PPK13561/cl32896, EAL domain containing protein | 297 | OrNV_gp028 |
| DuhNV_F3_ORF20 | ND | 232 | OrNV_gp029 |
| DuhNV_F3_ORF24 | None | 96 | no hit |
| DuhNV_F3_ORF25 | None | 157 | no hit |
| DuhNV_F3_ORF26 | 61k AcORF9 | 575 | OrNV_gp037 |
| DuhNV_F3_ORF27 | ND | 199 | OrNV_gp039 |
| DuhNV_F3_ORF28 | ND | 166 | OrNV_gp040 |
| DuhNV_F3_ORF29 | ND | 110 | OrNV_gp041 |
| DuhNV_F3_ORF32 | ND | 403 | OrNV_gp045 |
| DuhNV_F3_ORF33 | ND | 591 | OrNV_gp046 |
| DuhNV_F3_ORF34 | ND | 293 | OrNV_gp047 |
| DuhNV_F3_ORF35 5 | cl34121, energy-coupling factor ABC transporter | 490 | no hit |
| DuhNV_F3_ORF36 | None | 66 | no hit |
| DuhNV_F3_ORF37 | None | 278 | no hit |
| DuhNV_F3_ORF38 | ND | 592 | OrNV_gp132 |
| DuhNV_F3_ORF39 | None | 868 | no hit |
| DuhNV_F3_ORF40 | None | 85 | no hit |
| DuhNV_F4_ORF1 | None | 76 | no hit |
| DuhNV_F4_ORF3 | None | 616 | no hit |
| DuhNV_F4_ORF4 | ND | 91 | OrNV_gp104 |
| DuhNV_F4_ORF5 | ND | 408 | OrNV_gp105 |
| DuhNV_F4_ORF8 | None | 68 | no hit |
| DuhNV_F4_ORF11 | None | 120 | no hit |
| DuhNV_F4_ORF13 | ND | 566 | OrNV_gp114 |
| DuhNV_F4_ORF15 | ND | 345 | OrNV_gp116 |
| DuhNV_F4_ORF17 | ND | 266 | OrNV_gp118 |
| DuhNV_F4_ORF18 | ND | 454 | OrNV_gp119 |
| DuhNV_F4_ORF19 | ND | 1285 | OrNV_gp120 |
| DuhNV_F4_ORF21 | ND | 143 | OrNV_gp122 |
| DuhNV_F4_ORF22 | ND | 124 | OrNV_gp123 |
| DuhNV_F4_ORF23 | ND | 476 * | OrNV_gp124 |
Bold, baculovirus core genes; italic, common nudivirus genes. *, separate fragments of the same gene; ND, domain search not done for genes with Oryctes rhinoceros nudivirus (OrNV) homologs; none, no pfam domains identified. 1 YL1 nuclear protein; 2 cl29686, domain of unknown function (DUF4679); 3 chromosome segregation ATPase (cell cycle control, cell division, chromosome partitioning); 4 putative diguanylate cyclase (provisional); 5 ABC transporter protein.