Table 5.
Measured staphylococcal enterotoxins (SE) concentrations in culture supernatants from eight S. aureus strains grown in BHI medium. The supernatants were collected and processed as described in methods before quantification using the 3-h sequential format sandwich immunoassay with poly-horseradish peroxidase-labeled streptavidin detection. Concentrations were calculated with GraphPad Prism software using a nonlinear regression model (two-site binding saturation curve fit). Means and standard deviations for SE concentrations in supernatants were calculated from two experiments performed with two independent S. aureus cultures.
| mAb pair | Target SE | Measured SE Concentrations (ng/mL eq. Recombinant SE) in Culture Supernatant from Strain: | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 07HMPA50 | FRI S6 | A900322 | 16SBCL1204 | 15SBCL1428 | FRI 569 | 12CEB51 | 14SBCL881 | ||
| SEA7/SEA5-biot | SEA | <LoD | 0.18 ± 0.02 | <LoD | LoD | <LoD | <LoD | <LoD | <LoD |
| SEB27/SEB26-biot | SEB | <LoD | 13400 ± 2263 | <LoD | <LoD | <LoD | <LoD | <LoD | <LoD |
| SEG41/SEG27-biot | SEG | <LoD | <LoD | 2.6 ± 0.9 | 5.3 ± 1.6 | 3.3 ± 0.4 | <LoD | <LoD | <LoD |
| SEH1/SEH19-biot | SEH | <LoD | <LoD | <LoD | <LoD | <LoD | 103.6 ± 41.2 | 43.9 ± 14.4 | 82.7 ± 33.5 |
| SEI27/SEI126-biot | SEI | <LoD | <LoD | 8.1 ± 7.7 | 1.9 ± 0.6 | 5.7 ± 0.1 | <LoD | <LoD | <LoD |