Fig. 1.

Composition of the splenic B cell pool of young and old mice.The spleens of 9 pairs of young and old mice were stained to evaluate percentages of the major B cell subsets. Results show percentages of FO, ABCs and MZ B cells. Top. Gating strategies showing that cells are first gated on Live CD45+ to exclude dead cells and then on CD19+AA4.1-CD43- cells to exclude transitional (AA4.1+) and B1 (CD43+) B cells. Center. A representative dot plot of splenic B cells from one young and one old mouse. Bottom. Frequencies of FO, ABCs and MZ B cells. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). ****p<0.0001