Fig. 3.

Total B cells and ABCs from old mice secrete higher amounts of IgG antibodies with autoimmune specificities as compared to those from young mice. a. B cells were stimulated for 7 days with CpG, then the supernatants were collected and IgG antibodies specific for MDA (left) and for adipocyte-derived antigens (right) were measured by ELISA. Results show OD of MDA-specific IgG/total IgG and adipocyte-specific IgG/total IgG, respectively. Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). b. IgG secretion in cultures of CpG-stimulated FO and ABCs sorted from the spleens of young and old mice. Results are expressed as ratios of MDA-specific/total IgG (left) and adipocyte-specific IgG/total IgG (right). Mean comparisons between groups were performed by two-way ANOVA. *p<0.05, **p<0.01, ****p<0.0001