Fig. 6.

Total B cells and ABCs from old mice are characterized by higher mRNA expression of enzymes involved in metabolic pathways as compared to those from young mice. Left. A diagram depicting the two major pathways analyzed is shown, with the enzymes used to measure anaerobic glycolysis (LDHA) and OXPHOS (PDHX) in parentheses. Center. B cells, isolated by magnetic sorting from the spleens of young and old mice, were left unstimulated, the mRNA was extracted and qPCRperformed to evaluate RNA expression of LDHA (top) and PDHX (bottom). Mean comparisons between groups were performed by unpaired Student’s t test (two-tailed). Right. RNA expression of LDHA (top) and PDHX (bottom) in unstimulated FO and ABCs sorted from the spleens of young and old mice. Mean comparisons between groups were performed by two-way ANOVA. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001