Fig. 1.

Effect of α-Mangostin on CHIKV as assessed by focus forming unit assay (a) and qRT-PCR assay (b) under pretreatment, cotreatment and posttreatment conditions at 24 h. Vero E6 cells were pre, co and posttreated with different concentrations (1, 2, 4 and 8 µM) of α-Mangostin. After 24 h incubation, the plates were freezed and the culture filtrates were used for the different assays. For the FFU assay, mouse anti-chikungunya antibody and goat anti mouse IgG HRP conjugate as primary and secondary antibodies were used. For qRT-PCR total RNA was isolated and CHIKV RNA was detected by measuring E3 RNA copies by real-time RT-PCR. The titres are presented as log₁₀ titres ***p < 0.001; *p < 0.05. All the values are expressed as mean ± SD. The experiments were performed in triplicates in three independent trials. ***p < 0.001; **p < 0.01 and *p < 0.05 vs. vehicle control