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. 2021 Feb 9;11(2):459. doi: 10.3390/ani11020459

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cyprinid herpesvirus (CyHV)-3 loop-mediated isothermal amplification (LAMP) assay, test named KHV1. (A) Isothermal amplification of CyHV-3 DNA at different reactable temperatures from 60 °C to 67 °C. (B) Anneal derivative of the isothermal amplified products in the LAMP reaction showed in “A”. (C) Analytical sensitivity of CyHV-3 LAMP assay. The amplification graph shows serial dilutions from 10⁷ to 1 copy of a recombinant plasmid. (D) Anneal derivative of the isothermal amplified products in the LAMP reaction showed in “C”.