Table 2.
Cyprinid herpesvirus (CyHV)-3 loop-mediated isothermal amplification (LAMP) results of 174 archived DNA samples extracted from gill tissues of various fish species. №: the number of samples tested for each group. CyHV-1: carp pox virus; CyHV-2: herpesviral hematopoietic necrosis virus; AngHV: eel herpesvirus; HV: uncharacterized herpesvirus. MT: LAMP product melting temperature. NA: no applicable. LAMP interpretation column shows the number of LAMP false positives and false negatives per group.
| Pathogen | № | LAMP Amplification | MT | LAMP Interpretation | |
|---|---|---|---|---|---|
| False-Positive | False-Negative | ||||
| CyHV-3 | 85 | 76 | Correct | NA | 9 (˂LOD) |
| CyHV-3 variant | 30 | 0 | NA | 0 | NA |
| CyHV-1 | 28 | 3 | Different in 2 out of 3 | 1 | NA |
| CyHV-2 | 10 | 3 | Same as CyHV3, coinfection confirmed in one sample | 2 | NA |
| AngHV | 2 | 1 | Different | 0 | NA |
| HV | 2 | 1 | Same as CyHV-3 | 1 | NA |
| Negative samples | 47 | 0 | NA | 0 | NA |