Figure 5.

AtEXPA4 or AtEXPB5 can complement the slow pollen tube growth. (A,B), qRT-PCR analysis of AtEXPA4 (A) or AtEXPB5 (B) expression in 35-d-old inflorescences from atexpa4expb5, atexpa4expb5^A4OE, and atexpa4expb5^B5OE. BETA-TUBULIN4 was used as the reference gene, and expression in atexpa4expb5 was normalized to 1. The values are the mean ± SD, three biological replicates with three technical replicates in each biological replicate. (C) Aniline blue staining of pollen tubes at 3 h, 12 h, and 24 h after pollination, hours (h). Asterisks show positions where pollen tubes arrive, scale bars, 300 μm. (D) the percentage of pollen tube to pistil length in (C). Values are means, error bars are SD, n = 7 pistils per replicate, three biological replicates.