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. 2021 Feb 10;22(4):1755. doi: 10.3390/ijms22041755

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Quantification of Cx43 expression (a), mean number of Iba-1 positive cells/unit area (b) and GFAP immunoreactivity (c) in the saline, probenecid alone, tonabersat and probenecid combination treatment groups. Analysis revealed a significant decrease in Cx43, Iba-1 and GFAP in the treatment groups (a–c). The tonabersat and probenecid combination group further showed significantly fewer microglia compared to the probenecid group (b). Statistical analysis was conducted using one-way ANOVA, followed by Tukey’s multiple comparisons test. Significant values in comparison with results from the saline group are indicated with asterisks: *p < 0.05; ***p <0.001.