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Effect of FK866 on the H2O2-induced upregulation of SASP factors in hDPCs. The hDPCs were pretreated with FK866 (10 µM) for 2 h and then stimulated with H2O2 (400 nM) for 24 h. (A) The cell lysates were subjected to RT-PCR for determining the mRNA levels of the indicated SASP markers. GAPDH was used as an internal control. (B) Densitometric analysis for assessing the relative mRNA expression level of each SASP factor normalized to the mRNA level of GAPDH. ** p < 0.01, *** p < 0.001.
