Figure 10.

Interaction between living Candida albicans cells and FITC-labeled K10S. Confocal microscopy images of yeast cells incubated with the labeled peptide for 15 and 100 min are shown in (B,C), respectively. K10S was mainly bound to yeast cell wall. In (A), the same field is shown in light transmission. After 150 min, K10S entered viable yeast cells: (D) FITC; (E) PI; (F) merge of (D,E). Intensely fluorescent dead cells were observed after 180 min, as demonstrated by PI internalization: (G) FITC; (H) PI; (I) merge of (G,H). Bar, 5 μm.