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. 2021 Feb 10;22(4):1772. doi: 10.3390/ijms22041772

Figure 4.

Figure 4

Detection limit of the modified RT-LAMP-coupled toehold reaction on contrived samples. The modified RT-LAMP is performed for 60 min with different combinations of loop primers (Loop B and Loop F). The resulting RT-LAMP product was applied to the subsequent toehold reaction. Fold change was calculated as the absorbance value from the toehold reaction with trigger RNA divided by that without the trigger RNA at 60 min. (a) Schematic illustration of the modified RT-LAMP and the subsequent toehold reaction. The paper assay image was the result of Figure 4d for sensor M6. (b) Effect of loop primers to improve detection limit of sensor M6. (c) Effect of different combinations of loop primers to detection limit of sensor S1. (d) Compatibility of human saliva with RT-LAMP-coupled toehold reaction. Loop B and Loop F were used for sensor M6 and S1. Two-tailed student’s test; * p < 0.05, ** p < 0.01, *** p < 0.001; Error bars represent ± s.d., n = 3.