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. 2021 Feb 10;22(4):1771. doi: 10.3390/ijms22041771

Figure 4.

Figure 4

H3K9 acetylation was increased at the LVH stage, but H3K122 acetylation was increased at the HF stage. At 6 weeks of age, male Dahl salt-sensitive (DS) and salt-resistant (DR) rats were given an 8% NaCl diet for the following 6 or 15 weeks. Using echocardiography, left ventricular hypertrophy (LVH) stage was confirmed at 12 weeks of age, and heart failure (HF) stage was confirmed at 21 weeks of age. (A–C) mRNA was extracted from the hearts of DR and DS rats at 12 or 21 weeks of age, and mRNA levels of ANF (A), BNP (B), and β-MHC (C) were measured. (D–F) Nuclear proteins and histone fraction were extracted from the hearts of DR and DS rats at 12 or 21 weeks of age. (D) is a photograph of a representative Western blot, and (E,F) are quantifications (D). (G–L) In vivo ChIP assay was performed using heart lysates of DR and DS rats at 12 or 21 weeks of age with anti-acetyl-histone H3K9 antibody (G–I), anti-acetyl-histone H3K122 antibody (J–L), or normal rabbit IgG as a negative control (not detected). (A–C and E–L), N = 3; two-way ANOVA followed by Tukey test. * p < 0.05, † p < 0.01.