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. 2021 Feb 10;13(4):740. doi: 10.3390/cancers13040740

Figure 1.

Figure 1

Effects of toll-like receptor 7 (TLR7) modulation in non-small cell lung cancer (NSCLC) cells on functional angiogenic responses. Human umbilical vein endothelial cells (HUVECs) were cultured in the presence of cell culture conditioned media (CM) from A549 shCTR (a mass population), shTLR7 (two clones), and shFPR1 (two clones) (A), or CM from A549 or HOP62 treated or untreated (NT) with imiquimod (1 μg/mL) (10× and 20× magnifications) (B) in a 24-well plate. After 3 days, cells were fixed with ice-cold 70% ethanol, and tubule formation was evaluated. Sample images (A,B) and a quantification of the angiogenic response (C) are reported. Data are represented as mean ± SD of three independent experiments. * p < 0.05 compared to the control. (D) Cell proliferation assessed as BrdU incorporation of endothelial cells (HUVEC and human primary lung microvascular endothelial cell (HPLMEC)) cultured for 36 h in CM of A549 shCTR (a mass population) and shTLR7 (2 clones). Data are represented as mean ± SD of three independent experiments. * p < 0.05 compared to the control. (E) Percent of HUVEC migrated cells toward CM from A549 shCTR (a mass population) and shTLR7 (two clones) or from A549 and HOP62 cells treated or not with imiquimod (1 μg/mL). Data are represented as mean ± SD of three independent experiments. * p < 0.05 compared to the control.