Figure 4.

Mitochondrial uncoupling induces mitochondrial fragmentation independently of canonical fission/fusion protein expression. Representative images of PMHs stained with MTG treated with FCCP with or without Mdivi (A) and quantitation of mitochondrial length under these conditions (B). Mitochondrial membrane potential measured by TMRM staining after FCCP (C) and representative Western blots examining Mfn2 and Drp1 levels after FCCP treatment (D) and their densitometric quantitation (E). Error bars represent mean ± SEM for n = 3 mice. *p < .05 compared with control.