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. 2021 Feb 10;11(2):473. doi: 10.3390/ani11020473

Figure 2.

Figure 2

Effect of 15 μg/mL adiponectin supplementation during in vitro culture of somatic cell nuclear transfer (SCNT) embryos on the protein expression of XBP1 in ≥4-cell embryos on day-2 (a) and in blastocysts (b); Hoechst (blue) and immunofluorescence (green) staining of control and adiponectin-treated ≥4-cell embryos (scale bars 200 μm) (c) and blastocysts (scale bars 100 μm) (d). Comparison of XBP1 expression in the blastocyst stage derived from parthenogenetic activation and SCNT (scale bars 100 μm) (e,f). At least 24 embryos per group from 4 biological replicates were analyzed in each experiment. Data are expressed as mean ± SD. a,b Values with letters indicate significant difference (p < 0.05). The experiment was performed as at least three independent replicates. AdipoQ 15 = adiponectin 15 μg/mL.