Figure 3.

Chondrogenic, adipogenic and osteogenic differentiation of hADSCs. Prior to image analysis by phase contrast microscopy, cells were differentiated as follows. Chondrogenic differentiation was determined by staining with Alcian blue on day 21 after seeding. For adipogenic differentiation, native and IL2-genetically modified cells were cultured in the reprogramming medium for 14 days. At day 14, cells were fixed and stained with Oil Red O. For osteogenic differentiation, cells were cultured in the reprogramming medium for 28 days. At day 28, cells were fixed and analyzed by von Kossa staining (chondrogenic and adipogenic differentiation: scale bar, 200 µm osteogenic differentiation: scale bar, 100 µm).