Figure 6.

(a) Gating strategy for flow cytometry of immune cells to analyse the following populations: CD3⁺ CD8⁺ CD4⁻ CD38⁺ HLA-DR⁺ T-killers, CD3⁻CD56⁺ NK cells, CD3⁺CD4⁺CD8⁻CCR6⁻CXCR3⁺ Th1 cells, CD3⁺CD4⁺CD8⁻CCR6⁻CXCR3⁻ Th2 cells, CD3⁺CD4⁺CD8⁻CCR6⁺CXCR3⁻ Th17 cells, CD3⁺CD4⁺CD8⁻CD25⁺CD127^low Tregs. (b) The cultivation of CD8⁺ T-cells with hASDCs-IL2 and CIMVs-IL2 led to a significant increase in the number of activated CD38⁺HLA-DR⁺ T-killers. However, the cultivation of peripheral blood mononuclear cells (PBMCs) with CIMVs-IL2 led to a more significant (2-fold) increase in the number of activated T-killers compared to hADSCs-IL2 cells (by 1.3 times). The number of NK cells did not increase after culturing with hADSCs, and decreased after incubation with CIMVs. Small changes (both an increase and a decrease) in the number of Th cells were observed in various samples, both native and genetically modified. At the same time, CIMVs-IL2 did not increase the number of regulatory T-cells (Tregs), unlike hADSCs-IL2. Each box represents the mean ± SD (n = 4) of four biological replicates, the data were calculated as relative to control PBMCs. **p < 0.01, ***p < 0.001, ****p < 0.0001.