Figure 5.

AC16 cells cultured under higher oxygen concentration (higher oxidative stress) display some similar protein signatures as failing human hearts. (A). Volcano plot of differentially expressed proteins between normoxic (N) and physioxic conditions (harvested under physioxia, PP); (B). Volcano plot of differentially expressed proteins between cells cultured in physioxia but harvested in normoxia (PN) and cells cultured and harvested under physioxia (PP). (C). Gene-ontology enrichment of biological process (GOBP) with significantly more abundant proteins in normoxia (N) compared to physioxia (PP) (proteins used for input must have passed the significance threshold of false discovery rate (FDR)-corrected p-value < 0.05 and have a fold change (compared to physioxia) >1.3). N = 3 per condition, FDR.