Figure 7.

Overlay images of U2OS cells treated with compounds 4 ((a): 1 µg/mL; (b): 5 µg/mL; (g): 5 µg/mL, wash-out), 7 ((c): 1 µg/mL; (d): 5 µg/mL; (h): 5 µg/mL, wash-out) or vehicle control (DMSO, (e): 5 µl/mL; (f): wash-out control), PFA-fixed and stained for their actin cytoskeleton (phalloidin-ATTO594, red) and nuclei (DAPI, blue). Knot-like structures and aggregates of f-actin in treated cells demonstrate the disruptive effect of compounds 4 and 7 in comparison to the intact actin cytoskeleton depicted in the vehicle control (e). Note that the effect of 4 is not reversible, as a wash-out (f) with a recovery time of one hour did not lead to a regeneration of the f-actin network (compare (b,g)), while the effect conveyed by compound 7 was fully revertible (compare (d) and (h)). Scales bars set as 20 µm.