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. 2021 Feb 15;8:625016. doi: 10.3389/fcvm.2021.625016

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Copyright © 2021 Kennedy, Brown, Abutaleb and Truskey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Monolayer method of EC differentiation from iPSCs using small molecules. iPSCs transition to lateral mesoderm through addition of CHIR99021, BMP4, and FGF2 for 3 days. Endothelial specification is induced for 3 to 5 days using VEGF-A, SB431542, and forskolin. Sorting for CD144⁺ and CD31⁺ cells generates a pure differentiated EC population.