Figure 2.

Intrinsic motility of SMG T_RM triggered by environmental topography. (A) Model for autonomous exocrine gland T_RM motility generated by baseline retrograde F-actin flow coupled via non-specific substrate friction or low adhesiveness under physical confinement. In addition, under completely non-adhesive conditions, cell propulsion can be generated through bending of the retrograde cortical actin flow by the environmental topography. Adapted from Reversat et al. (88). (B) Exemplary track of isolated SMG T_RM in “under agarose” confinement on human serum albumin with and without pluronic acid (PA) passivation to abolish residual friction or lodged between 7 μm-polystyrene bead clusters. Scale bar, 20 μm. (C) T_RM speeds within or outside of polystyrene bead clusters in presence of PA. (D) T_RM meandering index within or outside of polystyrene bead clusters in presence of PA. Data were analyzed by unpaired t-test (C) or Mann–Whitney test (D). *p < 0.05; **p < 0.01.